For Fudan University, we constructed an antibiotic-treated (ABX) pseudo-germ-free colonization mouse model, and the related findings revealed how gut lactic acid and its key microbial mediators alleviate anxiety (published in Gut Microbes).

Original title：Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila

Journal：Gut Microbes

Impact factor：11

In this study, antibiotic-treated (ABX) pseudo-germ-free mice were used to conduct key causal validation experiments to demonstrate the role of gut microbiota in tryptophan metabolism and anxiety regulation. (Conducted by Gnotobio Biotechnology)

Research results presentation：

In ABX-treated mice, mono-colonization with A. muciniphila or combined supplementation with lactic acid was performed. Supplementation with A. muciniphila, particularly in combination with lactic acid, restored metabolic balance, enhanced 5-HT activity, and alleviated anxiety, demonstrating the specific regulatory role of the lactic acid-A. muciniphila axis.